|
KMID : 0352519820190030663
|
|
Korea Univercity Medical Journal
1982 Volume.19 No. 3 p.663 ~ p.669
|
|
|
The propagation of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in tissue culture cells
|
|
|
|
|
Abstract
|
|
|
Korean hemorrhagic fever (KHF) was recognized for the first time in 1951 among United Nations troops and has remained endemic near the demilitarized zone between North and South Korea. But, recently it appears to have spreaded slowly all over the World.
In 1976 Lee & Lee succeeded in demonstrating an antigen in the lungs of the striped field mouse, Apodemus agrarius, which gave specific immunofluorescent antibody reactions with sera from patients convalescent from KHF for the first time. In 1981 Lee et al. registered this antigen as Hantaan virus. Recently, French et al. reported a successful growth of the virus in A549 cells. This is the successful results on growth curve after inoculation of Hantaan virus into A549 cells which originated from alveoler epithelial cells of human lung cancer. The results are summerized as follows.
1. The propagation of Hantaan virus could be demonstrated in the cytoplasm of A549 cells on 3rd day after inoculation of the virus, about 60% of cells were infected on 5th day, 80% on 6th day and 100% on 8th day. Cytopathic effect was not appeared.
2. The viral antigens were appeared as a fine spots in the cytoplasm of cells by in-direct immunofluorescent antibody technique and at times as a granule-like morphology.
3. The infected A549 cells with Hantaan virus can be used as an antigen for serologic diagnosis of KHF and there was no occurence of nonspecific reaction.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|